DEVELOPMENT OF EMBRYONIC STRUCTURES FROM ISOLATED MOUSE BLASTOMERES

Postimplantation development of blastomeres isolated from 4- and 8-cell mouse eggs.

Blastomeres isolated from 4- and 8-cell mouse eggs were inserted into empty zonae and transferred to the oviduct. The products of both types of blastomere were capable of inducing decidual formation. One implant produced by an isolated blastomere from a 4-cell egg contained a small, retarded embryo at 5 1/2 days but most decidua from blastomeres of either 4- or 8-cell eggs contained only a few ...

Mouse singletons and twins developed from isolated diploid blastomeres supported with tetraploid blastomeres.

The aim of this study was to obtain mice, hopefully identical multiplets, from single diploid blastomeres isolated at the 4-cell stage, or from pairs of sister blastomeres isolated at the 8-cell stage. To this end isolated blastomeres were aggregated with one or two tetraploid carrier embryos produced by electrofusion of 2-cell embryos. Diploid embryos were albino and homozygous for the "a" all...

Development of blastomeres of mouse eggs isolated at the 4- and 8-cell stage.

Identical triplets and twins developed from isolated blastomeres of 8- and 16-cell mouse embryos supported with tetraploid blastomeres.

We studied the developmental potential of single blastomeres from early cleavage mouse embryos. Eight- and sixteen-cell diploid mouse embryos were disaggregated and single blastomeres from eight-cell embryos or pairs of sister blastomeres from sixteen-cell embryos were aggregated with 4, 5 or 6 tetraploid blastomeres from 4-cell embryos. Each diploid donor embryo gave eight sister aggregates, w...

The development of isolated blastomeres in Bithynia tentaculata (Prosobranchia, Gastropoda).

The development of isolated blastomeres, separated at the first and second cleavage has been studied. After separation at the first cleavage, one half-embryo (CD) becomes swollen and balloon-like and develops adult organs such as eyes, tentacles, shell, operculum, etc. The other half (AB) is much more compact and never develops these adult structures. After separation on the alternate axis at t...